Stacy Malaker

Research

The overarching goal of my laboratory is to develop methods that allow for mass spectrometry (MS) analysis of mucin-domain glycoproteins, a class of densely O-glycosylated extracellular proteins. We will also study mucins in a biological context, since these proteins are integral in numerous diseases including: cancer, cystic fibrosis, and inflammatory bowel disease (IBD).  My expertise in MS instrumentation and data analysis, combined with my experience in chemical biology and glycobiology, gives my laboratory a unique ability to tackle long-standing challenges associated with glycoproteomics. These studies will ultimately allow us to unravel the complex relationship between mucins and human disease.

Education

B.S. University of Michigan, 2009
Ph.D. University of Virginia, 2014
Postdoctoral Scholar, Stanford University, 2016-2020

Honors

• University of Michigan Honors, 2007-2009
• University of Michigan Angell Scholar, 2009
• Alpha Chi Sigma Presidential Award, 2010
• University of Virginia Chemistry Department Fellowship, 2010-2013
• Pratt Fellowship, 2013
• Huskey Research Symposium Poster; First Place, 2013
• Robert J. Huskey Travel Fellowship Recipient, 2014
• Washington-Baltimore Mass Spectrometry Discussion Group Travel Award, 2014
• Huskey Research Symposium Oral Presentation; First Place, 2014
• University of Virginia 3 Minute Thesis; First Place, 2014
• Achievement Rewards for College Scientists (ARCS), Danaher Scholar, 2013-2014
• University of Virginia Post Doc Symposium Oral Presentation; Third Place, 2015
• University of Virginia CGS/ProQuest Distinguished Dissertation Nominee, 2015
• National Research Council (NRC) Research Associateship Award (Declined), 2016
• Stanford University Mass Spectrometry Seed Grant, 2016
• Helena Anna Henzl-Gabor Young Women in Science Travel Grant, 2017
• NIH T32 Stanford School of Medicine Immunology Training Grant (Declined), 2017-2018
• NIH F32 Ruth L. Kirschstein National Research Service Award (NRSA), 2017-2019
• France-Stanford Center for Interdisciplinary Studies Visiting Junior Scholar Fellowship, 2018
• SUMS Research Symposium Poster; First Place, 2018
• Helena Anna Heenzl-Gabor Young Women in Science Travel Grant, 2018
• Gordon Research Conference in Glycobiology; Poster Award, 2019
• Society for Glycobiology Annual Conference Travel Award, 2019
• Human Proteome Organization Early Career Researcher Manuscript Competition – Finalist, 2021
• Royal Society for Chemistry - Chemistry Biology Interface Division Horizon Prize, 2021           
• American Society for Biochemistry and Molecular Biology (ASBMB) Early Career Faculty Award, 2021
• Rising Stars in Proteomics and Metabolomics: 40 under 40, 2021
• Wendy U. and Thomas C. Naratil Pioneer Award, 2023
• American Society for Mass Spectrometry Research Award, 2023
• Human Proteome Organization Early Career Researcher Manuscript Competition – Finalist, 2024
• International Glycoconjugate Organization Hakomuri Young Glycoscientist Award, 2025

Recent Publications

Google Scholar

Keira E. Mahoney, Vincent Chang, Taryn M. Lucas, Krystyna Maruszko, and Stacy A. Malaker*. Mass Spectrometry-Compatible Elution Technique Enables an Improved Mucin-Selective Enrichment Strategy to Probe the Mucinome. Analytical Chemistry. 2024, 96(13):5242-5250. doi.org/10.1021/acs.analchem.3c05762

Joann Chongsaritsinsuk‡, Alexandra D. Steigmeyer‡, Keira E. Mahoney‡, Mia A. Rosenfeld‡, Taryn M. Lucas, Courtney M. Smith, Alice Li, Deniz Ince, Fiona L. Kearns, Alexandria S. Battison, Marie A. Hollenhorst, D. Judy Shon, Katherine H. Tiemeyer, Victor Attah, Catherine Kwon, Carolyn R. Bertozzi, Michael J. Ferracane, Mark A. Lemmon, Rommie E. Amaro, and Stacy A. Malaker*. Glycoproteomic landscape and structural dynamics of TIM family immune checkpoints enabled by mucinase SmE. Nature Communications. 2023, 14: 6169. doi.org/10.1038/s41467-023-41756-y Human Proteome Organization (HUPO) Early Career Research Manuscript Finalist

Valentina Rangel-Angarita, Keira E. Mahoney, Catherine Kwon, Raibat Sarker, Taryn M. Lucas, and Stacy A. Malaker*. False positive glycopeptide identification via in-FAIMS fragmentation. JACS Au. 2023, 3(9): 2498–2509. doi.org/10.1021/jacsau.3c00264

Marie A. Hollenhorst, Katherine H. Tiemeyer, Keira E. Mahoney, Kazuhiro Aoki, Mayumi Ishihara, Sarah C. Lowery, Valentina Rangel-Angarita, Carolyn R. Bertozzi, and Stacy A. Malaker*. Comprehensive analysis of glycoprotein Ibα ectodomain glycosylation. Journal of Thrombosis and Haemostasis, 2023, 21(4): 995-1009. 

Beatriz Calle‡, Ganka Bineva-Todd‡, Andrea Marchesi‡, Helen Flynn, Mattia Ghirardello, Omur Y. Tastan, Chloe Roustan, Junwon Choi, M. Carmen Galan, Benjamin Schumann*, and Stacy A. Malaker*. Benefits of chemical sugar modifications introduced by click chemistry for glycoproteomic analyses. Journal of the American Society for Mass Spectrometry. 2021, 32(9): 3266-2375.

* = corresponding author
† = co-first author