Stacy Malaker

Member of Yale faculty since 2021

Research Interests

Research

The overarching goal of my laboratory is to develop methods that allow for mass spectrometry (MS) analysis of mucin-domain glycoproteins, a class of densely O-glycosylated extracellular proteins. We will also study mucins in a biological context, since these proteins are integral in numerous diseases including: cancer, cystic fibrosis, and inflammatory bowel disease (IBD). My expertise in MS instrumentation and data analysis, combined with my experience in chemical biology and glycobiology, gives my laboratory a unique ability to tackle long-standing challenges associated with glycoproteomics. These studies will ultimately allow us to unravel the complex relationship between mucins and human disease.

Professor Malaker explains research identifying biomarkers to detect cancerous ovarian cells earlier on NBC CT News.

Education

B.S. University of Michigan, 2009
Ph.D. University of Virginia, 2014
Postdoctoral Scholar, Stanford University, 2016-2020

Honors

University of Michigan Honors, 2007-2009
University of Michigan Angell Scholar, 2009
Alpha Chi Sigma Presidential Award, 2010
University of Virginia Chemistry Department Fellowship, 2010-2013
Pratt Fellowship, 2013
Huskey Research Symposium Poster; First Place, 2013
Robert J. Huskey Travel Fellowship Recipient, 2014
Washington-Baltimore Mass Spectrometry Discussion Group Travel Award, 2014
Huskey Research Symposium Oral Presentation; First Place, 2014
University of Virginia 3 Minute Thesis; First Place, 2014
Achievement Rewards for College Scientists (ARCS), Danaher Scholar, 2013-2014
University of Virginia Post Doc Symposium Oral Presentation; Third Place, 2015
University of Virginia CGS/ProQuest Distinguished Dissertation Nominee, 2015
National Research Council (NRC) Research Associateship Award (Declined), 2016
Stanford University Mass Spectrometry Seed Grant, 2016
Helena Anna Henzl-Gabor Young Women in Science Travel Grant, 2017
NIH T32 Stanford School of Medicine Immunology Training Grant (Declined), 2017-2018
NIH F32 Ruth L. Kirschstein National Research Service Award (NRSA), 2017-2019
France-Stanford Center for Interdisciplinary Studies Visiting Junior Scholar Fellowship, 2018
SUMS Research Symposium Poster; First Place, 2018
Helena Anna Heenzl-Gabor Young Women in Science Travel Grant, 2018
Gordon Research Conference in Glycobiology; Poster Award, 2019
Society for Glycobiology Annual Conference Travel Award, 2019
Human Proteome Organization Early Career Researcher Manuscript Competition – Finalist, 2021
Royal Society for Chemistry - Chemistry Biology Interface Division Horizon Prize, 2021
American Society for Biochemistry and Molecular Biology (ASBMB) Early Career Faculty Award, 2021
Rising Stars in Proteomics and Metabolomics: 40 under 40, 2021
Chemical and Engineering (C&E) News Talented 12, 2023
Wendy U. and Thomas C. Naratil Pioneer Award, 2023
American Society for Mass Spectrometry Research Award, 2023
Human Proteome Organization Early Career Researcher Manuscript Competition – Finalist, 2024

Recent Publications

Google Scholar

Keira E. Mahoney, Vincent Chang, Taryn M. Lucas, Krystyna Maruszko, and Stacy A. Malaker*. Mass Spectrometry-Compatible Elution Technique Enables an Improved Mucin-Selective Enrichment Strategy to Probe the Mucinome. Analytical Chemistry. 2024, 96(13):5242-5250. doi.org/10.1021/acs.analchem.3c05762

Joann Chongsaritsinsuk‡, Alexandra D. Steigmeyer‡, Keira E. Mahoney‡, Mia A. Rosenfeld‡, Taryn M. Lucas, Courtney M. Smith, Alice Li, Deniz Ince, Fiona L. Kearns, Alexandria S. Battison, Marie A. Hollenhorst, D. Judy Shon, Katherine H. Tiemeyer, Victor Attah, Catherine Kwon, Carolyn R. Bertozzi, Michael J. Ferracane, Mark A. Lemmon, Rommie E. Amaro, and Stacy A. Malaker*. Glycoproteomic landscape and structural dynamics of TIM family immune checkpoints enabled by mucinase SmE. Nature Communications. 2023, 14: 6169. doi.org/10.1038/s41467-023-41756-y Human Proteome Organization (HUPO) Early Career Research Manuscript Finalist

Valentina Rangel-Angarita, Keira E. Mahoney, Catherine Kwon, Raibat Sarker, Taryn M. Lucas, and Stacy A. Malaker*. False positive glycopeptide identification via in-FAIMS fragmentation. JACS Au. 2023, 3(9): 2498–2509. doi.org/10.1021/jacsau.3c00264

Marie A. Hollenhorst, Katherine H. Tiemeyer, Keira E. Mahoney, Kazuhiro Aoki, Mayumi Ishihara, Sarah C. Lowery, Valentina Rangel-Angarita, Carolyn R. Bertozzi, and Stacy A. Malaker*. Comprehensive analysis of glycoprotein Ibα ectodomain glycosylation. Journal of Thrombosis and Haemostasis, 2023, 21(4): 995-1009.

Beatriz Calle‡, Ganka Bineva-Todd‡, Andrea Marchesi‡, Helen Flynn, Mattia Ghirardello, Omur Y. Tastan, Chloe Roustan, Junwon Choi, M. Carmen Galan, Benjamin Schumann*, and Stacy A. Malaker*. Benefits of chemical sugar modifications introduced by click chemistry for glycoproteomic analyses. Journal of the American Society for Mass Spectrometry. 2021, 32(9): 3266-2375.

* = corresponding author
† = co-first author