Member of the Microbial Sciences Institute
Member of Yale faculty since 2017
Molecular interactions between microbial and host cells can profoundly impact human health and disease. My laboratory studies enzymes and other proteins that dynamically shape these interactions in the context of gastrointestinal infections. We use a technique called activity-based proteomics to identify proteins that are active in infected cells and animals using chemical probes designed to selectively label, enrich, and quantify reactive amino acids in complex proteomes. We are applying these probes to uncover secreted enzymes that mediate host-microbe interactions in the gut. We are also using probes that target redox-sensitive cysteines to understand how reactive oxygen species produced during bacterial infections influence intercellular signaling at the host-microbe interface through the oxidation of protein thiols. By chemically dissecting the functional proteome of bacterial infections, we aim to build a quantitative, molecular understanding of biochemical pathways that underlie the development of disease and generate new leads for therapeutic targets, activity-based diagnostics, and drug delivery systems.
B.S., Massachusetts Institute of Technology, 2005
Ph.D., University of California, Berkeley, 2010
Charles A. King Trust Postdoctoral Fellow, Harvard Medical School and Brigham and Women’s Hospital, 2011-2016
Phi Beta Kappa, MIT, 2005
Outstanding Graduate Student Instructor Award, UC Berkeley, 2008
Thomas C. Alber Science and Engineering for Global Health Fellowship, Henry Wheeler Center for Emerging and Neglected Diseases, 2010
Charles A. King Trust Postdoctoral Fellowship, The Medical Foundation, 2014-2016
Hatzios SK, Abel S, Martell J, Hubbard T, Sasabe J, Munera D, Clark L, Bachovchin DA, Qadri F, Ryan ET, David BM, Weerapana E, and Waldor MK. (2016) “Chemoproteomic profiling of host and pathogen enzymes active in cholera,” Nat Chem Biol 12: 268-274.
Hatzios SK, Baer CE, Rustad TR, Siegrist MS, Pang JM, Ortega C, Alber T, Grundner C, Sherman DR, and Bertozzi CR. (2013) “Osmosensory signaling in Mycobacterium tuberculosis mediated by a eukaryotic-like Ser/Thr protein kinase,” Proc Natl Acad Sci USA 110(52): E5069-77.
Hatzios SK, Ringgaard S, Davis BM, and Waldor MK. (2012) “Studies of dynamic protein-protein interactions in bacteria using Renilla luciferase complementation are undermined by nonspecific enzyme inhibition,” PLoS ONE 7(8): e43715.
Hatzios SK, Schelle MW, Holsclaw CM, Behrens CR, Botyanszki Z, Lin FL, Carlson BL, Kumar P, Leary JA, and Bertozzi CR. (2009) “PapA3 is an acyltransferase required for Polyacyltrehalose biosynthesis in Mycobacterium tuberculosis.” J Biol Chem 284: 12745-12751.
Hatzios SK, Iavarone AT, and Bertozzi CR. (2008) “Rv2131c from Mycobacterium tuberculosis is a CysQ 3’-phosphoadenosine-5’-phosphatase.” Biochemistry 47: 5823-5831.
- Chemical Biology