Please join Yale Chemistry for a thesis seminar with Michael Burke, Davis and Batista Groups.
Title: Microscopy Driven Investigation of Subcellular Organization
Summary: Heterogeneity is observed all dimensions of high-resolution single cell images. This is unsurprising as cells are never at equilibrium: their molecular populations, concentrations, and spatial distributions are constantly changing in time. While the complexity and size of images collected by modern microscopes continues to grow, many existing computational approaches are limited in their ability to process large datasets. In this dissertation I developed and applied methods to both fluorescence and optical photothermal infrared (OPTIR) spectroscopy. With fluorescence methods I studied how organelles impact protein interactions and function. With OPTIR, I investigated infrared spectral hallmarks of organelles, developed markers of subcellular health, and monitored responses to oxidative stress. Overall, it provides advances in label-free imaging for cell biology.
